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In biochemistry, phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor (serine hydrolase inactivator) commonly used in the preparation of cell lysates. PMSF does not inactivate all serine proteases. The effective concentration of PMSF is between 0.1 - 1 mM. The half-life is short in aqueous solutions (110 min at pH 7, 55 min at pH 7.5, and 35 min at pH 8, all at 25 °C). Stock solutions are usually made up in anhydrous ethanol, isopropanol, or corn oil and diluted immediately before use. C6H5CH2SO2F F.W. 174.20 - CAS #329-98-6 | |
Description: ImmunoHistoZyme is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. ImmunoHistoZyme digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2-8°C. DO NOT FREEZE Intended Use:Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC. Reagent: Ready-to-use ImmunoHistoZyme Reagent. | |
Description: Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2-8°C. DO NOT FREEZE Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC. Reagent: Ready-to-use Pepsin reagent available in 2 sizes: 15 and 100 ml | |
Description: Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens. Storage: 2-8°C. DO NOT FREEZE Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC. Reagent: Ready-to-use Pronase reagent available in 2 sizes: 15 and 100 ml. | |
Triton X-100 is a nonionic surfactant that has a hydrophilic polyethylene oxide chain and an aromatic hydrocarbon lipophilic or hydrophobic group. Solubility in water: Soluble Refractive index (nD): 1.490-1.494 Molar mass: 647 g mol−1 CAS Number: 9002-93-1 (Polyethylene glycol tert-octylphenyl ether) CAS #9002-93-1 Specific Gravity (H2O=1): 1.065 MSDS Storage : RT | |
A prepared solution for the cleaning of diamond knives. Technical Data Sheet : Diamond Knife Wash Instructions Unit : 500 ml | |
Used for whole-cell preparations. Unit : 500 ml | |
Extract from Porcine Pancreas Used for whole cell preparations Unit : 25g | |