{"id":290245,"date":"2020-04-17T19:09:27","date_gmt":"2020-04-17T18:09:27","guid":{"rendered":"https:\/\/www.deltamicroscopies.com\/produit\/pearses-method-for-phospholipids\/"},"modified":"2026-04-22T05:29:40","modified_gmt":"2026-04-22T04:29:40","slug":"pearses-method-for-phospholipids","status":"publish","type":"product","link":"https:\/\/www.deltamicroscopies.com\/en\/products\/pearses-method-for-phospholipids\/","title":{"rendered":"Pearse&#8217;s Method for Phospholipids"},"content":{"rendered":"<p>A new histochemical method is described for the polychromatic staining and identification of phospholipids and sulpholipids in tissue sections. It is an adaptation of a chromatographic method described previously (Kennedy &amp; Collier, 1962). Sections are stained in a mixture of Diamond Sky Blue B, Diamond Cyanine R and formalin, followed by differentiation in 2-morpholinoethanol and bromine water, counterstaining in Cresyl Fast Violet and decolorization in lactic acid.<\/p>\n<p>The interpretation of the final colours in the test tissues was checked by extraction and chromatography of their lipid content.<\/p>\n<p>The staining of diverse animal and plant tissues is described and illustrated in colour.<\/p>\n<h4><strong>Stain results:<\/strong><\/h4>\n<table class=\"chemtable\">\n<tbody>\n<tr>\n<td>Phospholipids:<\/td>\n<td>\u00a0<\/td>\n<td>Blue<\/td>\n<\/tr>\n<tr>\n<td>Nuclei and Nucleoli:<\/td>\n<td>\u00a0<\/td>\n<td>Red<\/td>\n<\/tr>\n<tr>\n<td>Early lipofuchsin, eosinophil granules, keratin,<br \/>keratohyalin, human elastic tissues:<\/td>\n<td>\u00a0<\/td>\n<td>Blue to Purple<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>References:<br \/>Pearse, A. G.E., J. Pathl. Bact., 70:554-557, 1995<br \/>Luna, L.G. (ed), Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd Ed., McGraw-Hill, c. 1968, p. 148.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>For the staining of phospholipids, early lipofuchsin, etc.<\/p>\n<table style=\"width: 61.2%; border-collapse: collapse;\">\n<tbody>\n<tr>\n<td style=\"width: 17.9%; text-align: center;\"><a href=\"https:\/\/www.emsdiasum.com\/microscopy\/technical\/msds\/26681-01.pdf\">MSDS 26516-01<\/a><\/td>\n<td style=\"width: 23.1%; text-align: center;\"><a href=\"https:\/\/www.emsdiasum.com\/microscopy\/technical\/msds\/26516-02.pdf\">MSDS 26516-02<\/a><\/td>\n<td style=\"width: 20.18%; text-align: center;\"><a href=\"https:\/\/www.emsdiasum.com\/microscopy\/technical\/msds\/26516-03.pdf\">MSDS 26516-03<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 14pt;\"><strong><a href=\"https:\/\/www.deltamicroscopies.com\/wp-content\/uploads\/2020\/04\/Pearses-Method-for-Phospholipids.pdf\">Technical Data Sheet<\/a><\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"featured_media":289291,"comment_status":"open","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false},"product_brand":[],"product_cat":[1259,1279,1315],"product_tag":[1316],"class_list":{"0":"post-290245","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-histology","7":"product_cat-prepared-staining-kits-histo","8":"product_cat-lipid-fat-en","9":"product_tag-pearses-method-for-phospholipids-en","11":"first","12":"instock","13":"taxable","14":"shipping-taxable","15":"purchasable","16":"product-type-variable"},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - 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