{"id":290320,"date":"2020-04-14T17:01:38","date_gmt":"2020-04-14T16:01:38","guid":{"rendered":"https:\/\/www.deltamicroscopies.com\/produit\/carstairs-method-for-fibrin-and-platelets\/"},"modified":"2023-12-13T20:46:28","modified_gmt":"2023-12-13T19:46:28","slug":"carstairs-method-for-fibrin-and-platelets","status":"publish","type":"product","link":"https:\/\/www.deltamicroscopies.com\/en\/products\/carstairs-method-for-fibrin-and-platelets\/","title":{"rendered":"Carstairs Method for Fibrin and Platelets"},"content":{"rendered":"

FIXATION:<\/strong> \u00a0 \u00a0 \u00a0 \u00a010% buffered neutral formalin for 48 hours or longer.<\/span><\/p>\n

TECHNIQUE:<\/strong> \u00a0 \u00a0 \u00a0 \u00a0Paraffin sections cut at 5\u00a0<\/span>\u03bc<\/span>m.<\/span><\/p>\n

SOLUTIONS:<\/span><\/strong><\/span><\/p>\n

Modified Weigert<\/span>\u2019<\/span>s Iron Hematoxylin<\/span><\/strong><\/span><\/p>\n

(See\u00a0<\/span>Gomori\u2019s One Step Trichrome Method)<\/span><\/p>\n

0.5% Acid Alcohol Solution<\/span><\/p>\n

(See\u00a0<\/span>Truant\u2019s Method for Acid Fast Bacilli)<\/span><\/p>\n

Picro-Orange G Solution<\/span><\/p>\n

Picric acid, saturated aqueous ————————————————- \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0100.0 ml<\/span><\/p>\n

Orange G, C.I. 16230 ———————————————————– \u00a0 \u00a0 \u00a0 \u00a01.0 gm<\/span><\/p>\n

Ponceau-Fuchsin Solution<\/span><\/p>\n

Acid fuchsin, C.I. 42685 ——————————————————- \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a00.5 gm<\/span><\/p>\n

Ponceau 2R (Ponceau Xylidine) C.I. 16150 ———————————— \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a00.5 gm<\/span><\/p>\n

Distilled water ——————————————————————- \u00a0 \u00a0 \u00a0 \u00a099.0 ml<\/span><\/p>\n

Acetic acid ——————————————————————— \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a01.0 ml<\/span><\/p>\n

*<\/span>Phosphomolybdic-Phosphotungstic Acid Solution<\/span><\/p>\n

Phosphomolybdic acid ———————————————————- \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a02.5 gm<\/span><\/p>\n

Phosphotungstic acid ———————————————————– \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a02.5 gm<\/span><\/p>\n

Distilled water ——————————————————————- \u00a0 \u00a0 \u00a0 \u00a0100.0 ml<\/span><\/p>\n

\u00a0 \u00a0 \u00a0 \u00a0*This solution is the same one used in Masson<\/span>\u2019<\/span>s trichrome stain.<\/span><\/p>\n

*<\/span>Aniline Blue Solution<\/span><\/p>\n

Aniline blue, C.I. 42755 ——————————————————– \u00a0 \u00a0 \u00a0 \u00a0– \u00a0 \u00a0 \u00a0 \u00a01.0 gm<\/span><\/p>\n

Distilled water ——————————————————————- \u00a0 \u00a0 \u00a0 \u00a040.0 ml<\/span><\/p>\n

Acetic acid ———————————————————————– \u00a0 \u00a0 \u00a0 \u00a00.8 ml<\/span><\/p>\n

\u00a0 \u00a0 \u00a0 \u00a0*This solution is the same one used in Masson<\/span>\u2019<\/span>s trichrome stain.<\/span><\/p>\n

Staining Procedure:<\/strong><\/span><\/em><\/p>\n

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