{"id":290389,"date":"2020-04-08T12:44:35","date_gmt":"2020-04-08T11:44:35","guid":{"rendered":"https:\/\/www.deltamicroscopies.com\/produit\/halls-method-for-bilirubin\/"},"modified":"2020-04-08T12:44:35","modified_gmt":"2020-04-08T11:44:35","slug":"halls-method-for-bilirubin","status":"publish","type":"product","link":"https:\/\/www.deltamicroscopies.com\/en\/products\/halls-method-for-bilirubin\/","title":{"rendered":"Hall’s Method for Bilirubin"},"content":{"rendered":"

Staining Procedure:<\/a><\/strong><\/span><\/p>\n

\n
    \n
  1. Deparaffinize and hydrate to distilled water.<\/span><\/li>\n
  2. Stain in Fouchet<\/span>\u2019<\/span>s reagent for 10 minutes.<\/span><\/li>\n
  3. Wash in running water, then in distilled water.<\/span><\/li>\n
  4. Stain in Van Gieson<\/span>\u2019<\/span>s stain for 2 minutes.<\/span><\/li>\n
  5. Dehydrate in 95% alcohol, three changes in Coplin jars.<\/span><\/li>\n
  6. Complete dehydration in absolute alcohol, three changes.<\/span><\/li>\n
  7. Clear in xylene, three or four changes.<\/span><\/li>\n
  8. Mount with synthetic resin.<\/span><\/li>\n<\/ol>\n<\/div>\n

    \u00a0<\/h4>\n

    Stain results:<\/strong><\/h4>\n\n\n\n\n\n
    Biliverdin:<\/td>\n\u00a0<\/td>\nGreen<\/td>\n<\/tr>\n
    Collagen:<\/td>\n\u00a0<\/td>\nRed<\/td>\n<\/tr>\n
    Muscle:<\/td>\n\u00a0<\/td>\nYellow<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    References:
    Hall, M.J., Amer. J. Clin., 34:313 \u2013316, 1960
    Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Ed. McGraw-Hill, N.Y., p. 174, c. 1968<\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    PRINCIPLE<\/strong>: The oxidizing action of Fouchet’s reagent converts the bile pigment to green biliverdin (if it has been transported to the liver and reduced, it is referred to as\u00a0bilirubin<\/em>.). Colors range from olive green to emerald green, depending on the concentration of bile pigment present.<\/p>\n

    Technical Data Sheet<\/a><\/strong><\/p>\n

     <\/p>\n\n\n\n
    MSDS 26611-01<\/a><\/td>\nMSDS 26611-02<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"featured_media":289270,"comment_status":"open","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false},"product_brand":[],"product_cat":[1259,1332,1279],"product_tag":[1337],"class_list":{"0":"post-290389","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-histology","7":"product_cat-mineral-en","8":"product_cat-prepared-staining-kits-histo","9":"product_tag-halls-method-for-bilirubin-en","11":"first","12":"instock","13":"taxable","14":"shipping-taxable","15":"purchasable","16":"product-type-variable"},"yoast_head":"\nHall's Method for Bilirubin - Delta Microscopies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.deltamicroscopies.com\/en\/products\/halls-method-for-bilirubin\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Hall's Method for Bilirubin - Delta Microscopies\" \/>\n<meta property=\"og:description\" content=\"PRINCIPLE: The oxidizing action of Fouchet's reagent converts the bile pigment to green biliverdin (if it has been transported to the liver and reduced, it is referred to as\u00a0bilirubin.). Colors range from olive green to emerald green, depending on the concentration of bile pigment present.Technical Data Sheet MSDS 26611-01MSDS 26611-02 \" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.deltamicroscopies.com\/en\/products\/halls-method-for-bilirubin\/\" \/>\n<meta property=\"og:site_name\" content=\"Delta Microscopies\" \/>\n<meta property=\"og:image\" content=\"https:\/\/efb7cwj5g4q.exactdn.com\/wp-content\/uploads\/2020\/04\/image16730660581.jpg?strip=all&lossy=1&ssl=1\" \/>\n\t<meta property=\"og:image:width\" content=\"401\" \/>\n\t<meta property=\"og:image:height\" content=\"300\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Hall's Method for Bilirubin - Delta Microscopies","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.deltamicroscopies.com\/en\/products\/halls-method-for-bilirubin\/","og_locale":"en_US","og_type":"article","og_title":"Hall's Method for Bilirubin - Delta Microscopies","og_description":"PRINCIPLE: The oxidizing action of Fouchet's reagent converts the bile pigment to green biliverdin (if it has been transported to the liver and reduced, it is referred to as\u00a0bilirubin.). 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