{"id":291006,"date":"2020-03-27T09:20:48","date_gmt":"2020-03-27T08:20:48","guid":{"rendered":"https:\/\/www.deltamicroscopies.com\/produit\/bests-carmine-stock-solution\/"},"modified":"2024-08-02T09:53:22","modified_gmt":"2024-08-02T08:53:22","slug":"bests-carmine-stock-solution","status":"publish","type":"product","link":"https:\/\/www.deltamicroscopies.com\/en\/products\/bests-carmine-stock-solution\/","title":{"rendered":"Best’s Carmine Stock Solution"},"content":{"rendered":"
Best’s Carmine For Glycogen : <\/span><\/strong>Solutions<\/span><\/strong><\/div>\n
\n

Mayer’s hemalum or a similar nuclear stain<\/a>.<\/p>\n<\/div>\n

\n\n\n\n\n\n\n\n
Stock Solution<\/td>\n<\/tr>\n
Carmine<\/a><\/td>\n2.5<\/td>\ng<\/td>\n<\/tr>\n
Potassium carbonate<\/td>\n1.2<\/td>\ng<\/td>\n<\/tr>\n
Ammonia<\/td>\n25<\/td>\nmL<\/td>\n<\/tr>\n
Distilled water<\/td>\n75<\/td>\nmL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\n\n\n\n\n
\u00a0<\/td>\n<\/tr>\n
Combine the water, potassium carbonate and carmine. Boil for 15 minutes. Cool and filter. Add the ammonia.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\n\n\n\n\n\n\n
Working solution<\/td>\n<\/tr>\n
Stock solution<\/td>\n25<\/td>\nmL<\/td>\n<\/tr>\n
Methanol, absolute<\/td>\n35<\/td>\nmL<\/td>\n<\/tr>\n
Ammonia<\/td>\n35<\/td>\nmL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\n\n\n\n\n\n\n
Differentiator<\/td>\n<\/tr>\n
Ethanol, 95%<\/td>\n40<\/td>\nmL<\/td>\n<\/tr>\n
Methanol, absolute<\/td>\n20<\/td>\nmL<\/td>\n<\/tr>\n
Distilled water<\/td>\n50<\/td>\nmL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\n

Tissue sample
5\u00b5 paraffin sections of neutral buffered formalin fixed tissue are suitable. It is often recommended that an alcoholic fixative be used to preserve glycogen, but the majority may be demonstrated following formalin fixation. Alcohol fixation is not usually required except for critical applications.<\/p>\n

Method<\/p>\n

    \n
  1. Bring sections to water via xylene and ethanol.<\/li>\n
  2. Stain nuclei with hemalum. It is not necessary to blue.<\/li>\n
  3. Rinse with distilled water.<\/li>\n
  4. Place in the working solution overnight.<\/li>\n
  5. Rinse well with water.<\/li>\n
  6. Place in the differentiator until glycogen granules are prominent.<\/li>\n
  7. Dehydrate with ethanol.<\/li>\n
  8. Clear with xylene and mount with a resinous medium.<\/li>\n<\/ol>\n

    Expected results<\/p>\n