{"id":298422,"date":"2019-03-28T16:49:42","date_gmt":"2019-03-28T15:49:42","guid":{"rendered":"https:\/\/www.deltamicroscopies.com\/produit\/trumps-fixative\/"},"modified":"2019-03-28T16:49:42","modified_gmt":"2019-03-28T15:49:42","slug":"trumps-fixative","status":"publish","type":"product","link":"https:\/\/www.deltamicroscopies.com\/en\/products\/trumps-fixative\/","title":{"rendered":"Trump’s Fixative"},"content":{"rendered":"
Modified Trump\u2019s universal fixative<\/strong><\/span><\/em><\/pre>\n
\n
\n\n\n\n\n\n\n\n\n\n\n
Reagent<\/th>\nQuantity (for 40 mL)<\/th>\nFinal concentration<\/th>\n<\/tr>\n<\/thead>\n
Cacodylic acid<\/td>\n0.56 g<\/td>\n100 mM<\/td>\n<\/tr>\n
Paraformaldehyde (16%) (Electron Microscopy Sciences; sealed ampoules)<\/td>\n10 mL<\/td>\n4% (w\/v)<\/td>\n<\/tr>\n
Glutaraldehyde (8%) (Electron Microscopy Sciences; sealed ampoules)<\/td>\n5 mL<\/td>\n1% (v\/v)<\/td>\n<\/tr>\n
MgCl2<\/sub>\u00a0(1 M)<\/td>\n80 \u03bcL<\/td>\n2 mM<\/td>\n<\/tr>\n
\u00a0<\/td>\n<\/tr>\n
Add the cacodylic acid to ~20 mL of water. Adjust the pH to 7.2 with NaOH. Bring the volume to 25 mL with H2<\/sub>O. Add the paraformaldehyde, glutaraldehyde, and MgCl2<\/sub>. Make this fixative fresh immediately before using.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"
 <\/p>\n
A combination of Sodium Cacodylate, Formalin, and Glutaraldehyde. Ready to use.<\/p>\n
Storage : 0-5\u00b0C <\/strong><\/p>\n
MSDS<\/a><\/p>\n","protected":false},"featured_media":289146,"comment_status":"open","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"off","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false},"product_brand":[],"product_cat":[1362,1442,1354],"product_tag":[],"class_list":{"0":"post-298422","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-fixateurs-en","7":"product_cat-autres-en","8":"product_cat-reactifs-en","10":"first","11":"instock","12":"taxable","13":"shipping-taxable","14":"purchasable","15":"product-type-variable"},"yoast_head":"\nTrump's Fixative - Delta Microscopies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.deltamicroscopies.com\/en\/products\/trumps-fixative\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Trump's Fixative - Delta Microscopies\" \/>\n<meta property=\"og:description\" content=\" A combination of Sodium Cacodylate, Formalin, and Glutaraldehyde. 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