{"id":298529,"date":"2019-03-27T09:56:06","date_gmt":"2019-03-27T08:56:06","guid":{"rendered":"https:\/\/www.deltamicroscopies.com\/produit\/bouins-fixative\/"},"modified":"2025-08-10T19:44:51","modified_gmt":"2025-08-10T18:44:51","slug":"bouins-fixative","status":"publish","type":"product","link":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/","title":{"rendered":"Bouin’s Fixative"},"content":{"rendered":"

Bouin solution<\/b>, or\u00a0Bouin’s solution<\/b>, is a compound\u00a0fixative\u00a0used in\u00a0histology.<\/sup>\u00a0It was invented by French biologist\u00a0Pol Bouin<\/a>\u00a0and is composed of\u00a0picric acid,\u00a0acetic acid\u00a0and\u00a0formaldehyde\u00a0in an aqueous solution.\u00a0<\/sup>\u00a0Bouin’s fluid is especially useful for fixation of\u00a0gastrointestinal tract\u00a0biopsies because this fixative allows crisper and better\u00a0nuclear\u00a0staining\u00a0than 10% neutral-buffered formalin. It is not a good fixative when tissue\u00a0ultrastructure\u00a0must be preserved for\u00a0electron microscopy. However, it is a good fixative when tissue structure with a soft and delicate texture must be preserved. The acetic acid in this fixative\u00a0l<\/a>yses\u00a0red blood cells and dissolves small iron and calcium deposits in tissue.<\/p>\n

Variations Bouin<\/span><\/h2>\n

Gendre solution<\/span><\/h3>\n

Gendre solution is an alcoholic versi<\/span>on of Bouin solution. An alcoholic solution saturated with picric acid is used instead of an aqueous solution saturated with picric acid when making this solution. This solution is useful when glycogen and other carbohydrates must be preserved in tissue. It is prepared by a mixing saturated solution of picric acid in 95% ethanol (80ml) with formalin (37-40% formaldehyde) (15 ml) and glacial acetic acid (5ml).\u00a0<\/sup>\u00a0Gendre’s fixative contains more picric acid than Bouin’s, because of greater solubility (6.23%w\/v) in ethanol than in water (1.23%w\/v).<\/sup><\/p>\n

Hollande solution<\/span><\/h3>\n

Hollande solution is a version of Bouin solution that contains\u00a0copper acetate. The copper acetate stabilizes red blood cell membranes and the\u00a0granules\u00a0of\u00a0eosinophils\u00a0and\u00a0endocrine cells so that there is less lysis of these cell components than occurs in regular Bouin solution <\/sup>\u00a0Hollandes fixative is made by adding 6.25g cupric acetate, 10g picric acid (wet powder), 25ml formalin (37-40% formaldehyde) and 2.5 ml glacial (100%) acetic acid to 250ml water.<\/p>\n","protected":false},"excerpt":{"rendered":"

A fixative<\/strong> that is used in light microscopy<\/strong> for the preparation of delicate structure.<\/p>\n

is composed of\u00a0picric acid,\u00a0acetic acid\u00a0and\u00a0formaldehyde\u00a0in an aqueous solution.\u00a0<\/sup>\u00a0Bouin’s fluid is especially useful for fixation of\u00a0gastrointestinal tract\u00a0biopsies<\/strong><\/p>\n

Bouin solution<\/b>, or\u00a0Bouin’s solution<\/b>, is a compound\u00a0fixative\u00a0used in\u00a0histology.<\/sup><\/p>\n

Unit : 10 x 10ml<\/p>\n

Storage : RT<\/p>\n

MSDS<\/a><\/p>\n

 <\/p>\n","protected":false},"featured_media":287961,"comment_status":"open","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"off","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false},"product_brand":[],"product_cat":[1362,1259,1442,1354],"product_tag":[],"class_list":{"0":"post-298529","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-fixateurs-en","7":"product_cat-histology","8":"product_cat-autres-en","9":"product_cat-reactifs-en","11":"first","12":"instock","13":"taxable","14":"shipping-taxable","15":"purchasable","16":"product-type-simple"},"yoast_head":"\nBouin's Fixative - Delta Microscopies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Bouin's Fixative - Delta Microscopies\" \/>\n<meta property=\"og:description\" content=\"A fixative that is used in light microscopy for the preparation of delicate structure. is composed of\u00a0picric acid,\u00a0acetic acid\u00a0and\u00a0formaldehyde\u00a0in an aqueous solution.\u00a0\u00a0Bouin's fluid is especially useful for fixation of\u00a0gastrointestinal tract\u00a0biopsies Bouin solution, or\u00a0Bouin's solution, is a compound\u00a0fixative\u00a0used in\u00a0histology. Unit : 10 x 10ml Storage : RT MSDS  \" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/\" \/>\n<meta property=\"og:site_name\" content=\"Delta Microscopies\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-10T18:44:51+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.deltamicroscopies.com\/wp-content\/uploads\/2019\/03\/bouin-fixative-e1570955911192.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"400\" \/>\n\t<meta property=\"og:image:height\" content=\"276\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Bouin's Fixative - Delta Microscopies","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/","og_locale":"en_US","og_type":"article","og_title":"Bouin's Fixative - Delta Microscopies","og_description":"A fixative that is used in light microscopy for the preparation of delicate structure. is composed of\u00a0picric acid,\u00a0acetic acid\u00a0and\u00a0formaldehyde\u00a0in an aqueous solution.\u00a0\u00a0Bouin's fluid is especially useful for fixation of\u00a0gastrointestinal tract\u00a0biopsies Bouin solution, or\u00a0Bouin's solution, is a compound\u00a0fixative\u00a0used in\u00a0histology. Unit : 10 x 10ml Storage : RT MSDS  ","og_url":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/","og_site_name":"Delta Microscopies","article_modified_time":"2025-08-10T18:44:51+00:00","og_image":[{"width":400,"height":276,"url":"https:\/\/www.deltamicroscopies.com\/wp-content\/uploads\/2019\/03\/bouin-fixative-e1570955911192.jpg","type":"image\/jpeg"}],"twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/","url":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/","name":"Bouin's Fixative - Delta Microscopies","isPartOf":{"@id":"https:\/\/www.deltamicroscopies.com\/en\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/#primaryimage"},"image":{"@id":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/#primaryimage"},"thumbnailUrl":"https:\/\/efb7cwj5g4q.exactdn.com\/wp-content\/uploads\/2019\/03\/bouin-fixative-e1570955911192.jpg?strip=all","datePublished":"2019-03-27T08:56:06+00:00","dateModified":"2025-08-10T18:44:51+00:00","breadcrumb":{"@id":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/#primaryimage","url":"https:\/\/efb7cwj5g4q.exactdn.com\/wp-content\/uploads\/2019\/03\/bouin-fixative-e1570955911192.jpg?strip=all","contentUrl":"https:\/\/efb7cwj5g4q.exactdn.com\/wp-content\/uploads\/2019\/03\/bouin-fixative-e1570955911192.jpg?strip=all","width":400,"height":276,"caption":"Bouin's Fixative"},{"@type":"BreadcrumbList","@id":"https:\/\/www.deltamicroscopies.com\/en\/products\/bouins-fixative\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/www.deltamicroscopies.com\/en\/home-3\/"},{"@type":"ListItem","position":2,"name":"Our products","item":"https:\/\/www.deltamicroscopies.com\/en\/shop\/"},{"@type":"ListItem","position":3,"name":"Bouin’s Fixative"}]},{"@type":"WebSite","@id":"https:\/\/www.deltamicroscopies.com\/en\/#website","url":"https:\/\/www.deltamicroscopies.com\/en\/","name":"Delta Microscopies","description":"Des microscopistes \u00e0 votre service.","publisher":{"@id":"https:\/\/www.deltamicroscopies.com\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.deltamicroscopies.com\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.deltamicroscopies.com\/en\/#organization","name":"Delta Microscopies","url":"https:\/\/www.deltamicroscopies.com\/en\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.deltamicroscopies.com\/en\/#\/schema\/logo\/image\/","url":"https:\/\/efb7cwj5g4q.exactdn.com\/wp-content\/uploads\/2019\/09\/DELTAM_LOGO_RVB-1-1.png?strip=all&lossy=1&ssl=1","contentUrl":"https:\/\/efb7cwj5g4q.exactdn.com\/wp-content\/uploads\/2019\/09\/DELTAM_LOGO_RVB-1-1.png?strip=all&lossy=1&ssl=1","width":688,"height":177,"caption":"Delta Microscopies"},"image":{"@id":"https:\/\/www.deltamicroscopies.com\/en\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.instagram.com\/emsdiasum\/","https:\/\/www.youtube.com\/channel\/UCkSsQQq74DhyLXNg_9RPorQ"]}]}},"_links":{"self":[{"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/product\/298529","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/comments?post=298529"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/media\/287961"}],"wp:attachment":[{"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/media?parent=298529"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/product_brand?post=298529"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/product_cat?post=298529"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.deltamicroscopies.com\/en\/wp-json\/wp\/v2\/product_tag?post=298529"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}