{"id":302461,"date":"2020-01-13T14:48:13","date_gmt":"2020-01-13T13:48:13","guid":{"rendered":"https:\/\/www.deltamicroscopies.com\/produit\/cryocapsule\/"},"modified":"2020-01-13T14:48:13","modified_gmt":"2020-01-13T13:48:13","slug":"cryocapsule","status":"publish","type":"product","link":"https:\/\/www.deltamicroscopies.com\/en\/products\/cryocapsule\/","title":{"rendered":"CryoCapsule \u00ae"},"content":{"rendered":"

<\/a>CryoCapsule\u00ae<\/h3>\n

Bringing Correlative light and electron microscopy forward with the CryoCapsule\u00ae<\/em><\/p>\n

\"CryoCapsule\u00ae\"<\/em>High pressure freezing is the most advanced technology when it comes to vitrify a hydrated biological specimen while preserving the ultrastructure. Developed in the 80’s [1], the technology evolved progressively to become accessible to a larger community. Still, the sample preparation prior to HPF remains tedious and often comes to advance expertise depending on the specimen [2].<\/p>\n

The CryoCapsule\u00ae is a new tool in the field of High Pressure Freezing (HPF) and correlative light and electron microscopy (CLEM). Comparable to a small petri dish, it is composed of a landmarked sapphire disc and a gold spacer ring (50\u00b5m thick) maintained together by a plastic ring [3].<\/p>\n

https:\/\/youtu.be\/8PO3n9gKAa4?list=PLUd81CkPvzrAOlA1AkKNH327DH2BZuSoB<\/a><\/p>\n\n\n\n\n
\"CryoCapsule\u00ae<\/td>\n<\/tr>\n
CryoCapsule\u00ae 3mm, Catalog #3750<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

The specimens are encapsulated between the support sapphire disc (carbon landmarked) and a covering sapphire disc.<\/p>\n

The CryoCapsule\u00ae is loaded into a specific adaptor (HPM010, HPM100, HPF compact 02). Live cell imaging is done directly on the specimen in the CryoCapsule\u00ae prior to HPF.<\/p>\n\n\n\n\n
\"HPM010<\/td>\n\"HMP100<\/td>\n<\/tr>\n
HPM010 Abra Fluid Adapter, Catalog #3751<\/em><\/td>\nHMP100 Leica Adaptor, Catalog #3752<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Post-HPF, the specimen is processed for freeze substitution [4] and room temperature sectioning.<\/p>\n

CryoCapCell has developed a set of tools to manipulate the CryoCapsule\u00ae [4] in most scientific environments.<\/p>\n\n\n\n\n
\"D\u00e9capsuleur\"<\/td>\n\"D\u00e9capsuleur\"<\/td>\n<\/tr>\n
D\u00e9capsuleur, Catalog #3753<\/em><\/td>\nD\u00e9capsuleur Pencil, Catalog #3754<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/p>\n

    \n
  1. Moor H, Bellin G, Sandri C, Akert K. The influence of high pressure freezing on mammalian nerve tissue. Cell Tissue Res [Internet] 1980 [cited 2013 Jul 24];209:201-16.<\/li>\n
  2. Mcdonald KL, Schwarz H, Thomas M, M\u00fcller-Reichert T, Webb R, Buser C, Morphew M. “Tips and tricks” for high-pressure freezing of model systems. Methods Cell Biol [Internet] 2010 [cited 2011 Apr 19];96:671-93.<\/li>\n
  3. Heiligenstein X, Heiligenstein J, Delevoye C, Hurbain I, Bardin S, Paul-Gilloteaux P, Sengmanivong L, R\u00e9gnier G, Salamero J, Antony C, Raposo G. The CryoCapsule\u00ae: Simplifying Correlative Light to Electron Microscopy. Traffic [Internet] 2014 [cited 2014 May 14];15:700-16.<\/li>\n
  4. Heiligenstein X, Hurbain I, Delevoye C, Salamero J, Antony C, Raposo G. Step by step manipulation of the CryoCapsule\u00ae with HPM high pressure freezers. Methods Cell Biol [Internet] 2014 [cited 2014 Nov 27];124:259-74.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    High pressure freezing is the most advanced technology when it comes to vitrify a hydrated biological specimen while preserving the ultrastructure. Developed in the 80’s [1], the technology evolved progressively to become accessible to a larger community. Still, the sample preparation prior to HPF remains tedious and often comes to advance expertise depending on the specimen [2].<\/span><\/p>\n

     <\/p>\n","protected":false},"featured_media":288396,"comment_status":"open","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false},"product_brand":[],"product_cat":[1100,1106,1097],"product_tag":[],"class_list":{"0":"post-302461","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-cryo-methodes-en","7":"product_cat-cryocapsule-cryocapsule-en","8":"product_cat-cryocapsule-en","10":"first","11":"instock","12":"taxable","13":"shipping-taxable","14":"purchasable","15":"product-type-variable"},"yoast_head":"\nCryoCapsule \u00ae - Delta Microscopies<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.deltamicroscopies.com\/en\/products\/cryocapsule\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"CryoCapsule \u00ae - Delta Microscopies\" \/>\n<meta property=\"og:description\" content=\"High pressure freezing is the most advanced technology when it comes to vitrify a hydrated biological specimen while preserving the ultrastructure. 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