EMS Immuno Mount™ with anti-fading agent 1, 4-Diazobicyclo-(2,2,2-octane (DABCO™) is an aqueous non-permanent low viscosity mounting medium for immunofluorescence. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine and Redox. The fluorescence is retained during storage at 4°C in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI.
Not recommended for phycoethyrin (PE), phycocyanin (PC) and allophycocyanin (APC).
Application: Immunofluorescence, confocal microscopy
Reagent: Ready to use mounting medium for Immunofluorescence
Refractive Index: 1.400 ± 0.002 (These numbers apply to these mounting mediums in solution. Refractive indexes change when the water solvent evaporates and mounting media dries on slides. We do not have the means to measure the refractive indexes of dry mounting mediums; however, we expect the numbers to go higher when dried. The refractive index of water is 1.3330.)
Storage: 2-8°C, DO NOT FREEZE