For all of the best results we offer ready to use Perfusion washes and fixatives which are recommended by the NeuroScience Association. All of our solutions are ready made and are of the highest quality. We have three groups of solutions commercially available. Group I: Perfusion wash and Fixative Technical Grade. To be used for Standard stains: Perfusion Wash (1220SK): Phosphate Buffered Saline 01.M Phosphate Buffer pH 7.2-7.4 Perfusion Fixative (1221SK): 10% Phosphate BufferedFormaldehyde Group II: Perfusion wash and Fixative Reagent Grade. To be used for Standard and ImmunoHistochemistry Stains. Perfusion Wash (1219SK): Phosphate Buffered Saline 0.1M Phosphate Buffer pH 7.2-7.4 Perfusion Fixative (1224SK): Paraformaldehye 4%/Sucrose 4% in Phosphate Buffer 0.1M Ph 7.2 Group III: Perfusion wash and Fixative Superior Reagent Grade. For the best results use this group. This is Sodium cacodylate based for NSA’S Disintegrative Degeneration Stain. Perfusion Wash (1222SK): 0.8% NaCl/0.4% Dextrose/0.8% Sucrose/ 0.023% CaCl2 /Sodium. Cacodylate 0.034% Perfusion Fixative (1223SK): 4% Paraformaldehyde/4% Sucrose/ 1.4% Sodium Cacodylate. For a complete procedure and protocol for the use of these washes and fixatives please see the following site: www.neuroscienceassociates.com/perf-solution1 or 2 or 3.htm.
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Technical Tip The Fixative for Use in Fixing Cornea To fix cornea, one should use the following fixative: 2% Glutaraldehyde, 1% Paraformaldehyde (Formaldehyde) in a 0.1M Sodium Cacodylate buffer; pH 7.4 containg 0.02% Picric Acid. (Assouline, et al 1992)