Phosphate Buffer, 0.1M*

 

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only *Meant for biological research only. Not to be used for medical or any other purposes.

MSDS_19340-60     MSDS_19340-65

MSDS_19340-70       MSDS_19340-72    

REF: D19340 Categories: , , Tag:

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Description

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only *Meant for biological research only. Not to be used for medical or any other purposes.                   MSDS_19340-60                  MSDS_19340-65                 MSDS_19340-70           MSDS_19340-72    

info+

PB (0.1 M phosphate buffer pH 7.2)

Reagents

Sodium phosphate dibasic

Sodium phosphate monobasic (anhydrous)

Equipment

Beakers

Graduated cylinders

Hot plate (optional; see Step 1 note)

Scale

Spatulas

Stir plate

Weigh boats

METHOD

  • 1. Prepare sodium phosphate dibasic stock (0.5 M) by dissolving 35.5 g of sodium phosphate dibasic in a final volume of 500 mL of H2O.

    Some crystallization will occur when the solution is stored at 4ºC. Warm on a hot plate and stir until the crystals dissolve.

  • 2. Prepare sodium phosphate monobasic stock (0.5 M) by dissolving 30 g of anhydrous sodium phosphate monobasic in a final volume of 500 mL of H2O.

  • 3. Prepare 0.1 M sodium phosphate dibasic: Put 80 mL of sodium phosphate dibasic stock (0.5 M) from Step 1 in a beaker and add H2O to give a final volume of 400 mL.

  • 4. Prepare 0.1 M sodium phosphate monobasic: Put 30 mL of sodium phosphate monobasic stock (0.5 M) from Step 2 in a beaker and add H2O to give a final volume of 150 mL.

  • 5. Bring the 0.1 M sodium phosphate dibasic solution from Step 3 to pH 7.2 by adding as much as needed of the 0.1 M sodium phosphate monobasic solution from Step 4.

    The resulting solution is 0.1 M phosphate buffer pH 7.2.

Additional information

pH

pH 6.0, pH 6.5, pH 7.0, pH7.2

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