Tris Buffer pH 10.0 (10x)

Prepared from Sodium Cacodylate Trihydrate

his buffer is intended for heat-induced antigen retriever

(F.W. 214.02)

 

Storage : 0-5°C

REF: D64142-3 Categories: , ,

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Description

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.

Storage: 2-8°C. DO NOT FREEZE

Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol. Reagent: 10x, pH 10 antigen retriever solution.

Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C

MSDS_64142-11      

Additional information

Unit

100 ml, 1000 ml

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