Description
Modified Trump’s universal fixative
Reagent | Quantity (for 40 mL) | Final concentration |
Cacodylic acid | 0.56 g | 100 mM |
Paraformaldehyde (16%) (Electron Microscopy Sciences; sealed ampoules) | 10 mL | 4% (w/v) |
Glutaraldehyde (8%) (Electron Microscopy Sciences; sealed ampoules) | 5 mL | 1% (v/v) |
MgCl2 (1 M) | 80 μL | 2 mM |
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Add the cacodylic acid to ~20 mL of water. Adjust the pH to 7.2 with NaOH. Bring the volume to 25 mL with H2O. Add the paraformaldehyde, glutaraldehyde, and MgCl2. Make this fixative fresh immediately before using. |
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