Description
Modified Trump’s universal fixative
| Reagent |
Quantity (for 40 mL) |
Final concentration |
| Cacodylic acid |
0.56 g |
100 mM |
| Paraformaldehyde (16%) (Electron Microscopy Sciences; sealed ampoules) |
10 mL |
4% (w/v) |
| Glutaraldehyde (8%) (Electron Microscopy Sciences; sealed ampoules) |
5 mL |
1% (v/v) |
| MgCl2 (1 M) |
80 μL |
2 mM |
| |
| Add the cacodylic acid to ~20 mL of water. Adjust the pH to 7.2 with NaOH. Bring the volume to 25 mL with H2O. Add the paraformaldehyde, glutaraldehyde, and MgCl2. Make this fixative fresh immediately before using. |
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