Trump’s Fixative

 

A combination of Sodium Cacodylate, Formalin, and Glutaraldehyde. Ready to use.

Storage : 0-5°C

MSDS

REF: D1175 Categories: , ,

Available Options:

Description

Modified Trump’s universal fixative
Reagent Quantity (for 40 mL) Final concentration
Cacodylic acid 0.56 g 100 mM
Paraformaldehyde (16%) (Electron Microscopy Sciences; sealed ampoules) 10 mL 4% (w/v)
Glutaraldehyde (8%) (Electron Microscopy Sciences; sealed ampoules) 5 mL 1% (v/v)
MgCl2 (1 M) 80 μL 2 mM
 
Add the cacodylic acid to ~20 mL of water. Adjust the pH to 7.2 with NaOH. Bring the volume to 25 mL with H2O. Add the paraformaldehyde, glutaraldehyde, and MgCl2. Make this fixative fresh immediately before using.

Additional information

Unit

500ml, 4x500ml

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