Je m’appelle Augustin Larrieu, j’ai 13 ans et je suis collégien à Auterive (31190).
J’ai effectué mon stage d’observation du 1er au 5 février 2021 chez Delta Microscopies, une entreprise qui fabrique des outils pour les microscopes. Dans cette entreprise, pour entrer dans le laboratoire, il faut s’équiper d’ E.P.I ( Equipement de Protection Individuel) pour se proteger des substances dangereuses présentes dans la salle.
J’ai donc mis la blouse, les gants et une charlotte pour manipuler les produits sous controle d’un professionnel. Après avoir explorer le tableau de Mendeleïev, j’ai pu apprendre à préparer une solution: 500ml de Tampon Imidazole 0,2M pH 7,5. J’ai étudié sa formule, son poids moléculaire et sa molarité afin de préparer ce produit. Pour faire ce tampon, j’ai utilisé de la verrerie (bécher), du matériel et de de la poudre d’Imidazole que j’ai pesé et dissout dans 500ml d’eau distillée à l’aide d’un agitateur Magnétique. Il s’est formé une solution transparente d’un pH égal à 10,7. J ‘ai utilisé de l’acide chloridrique pour abaisser sa valeur. Après avoir ajuster son pH à 7,5 j’ai vidé la solution dans un flacon de 500ml. Le lendemain, j’ai créé et imprimé l’étiquette normalisée du tampon imidazole en spécifiant évidemment les pictogrammes de sécurité. J’ai ensuite donné le flacon à l’assistante logistique qui l’a emballé et expédié.
À la fin de mon stage j’ai pu m’entretenir avec la directrice de l’entreprise pour faire le bilan.
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