Immersion Oils Type HF

After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.

*1 oz. Applicator bottles have a glass ball rod in the cap.

Available Options:

Description

* Immersion Oil Type HF
Type DF has been discontinued by Cargille. As an alternative we are offering either a new Immersion Oil called HF which would provide low fluorescence and the correct optical properties or you can use our FF which has virtually no fluorescence but slightly less than ideal optical properties.

* Immersion Oil Type LDF
Extremely low fluorescence is achieved by Type LDF. It is the replacement of choice for the DF which is no longer being manufactured. The LDF possesses the highest resolution of the oils for fluorescence applications.

* Immersion Oil Type FF
Type FF has virtually no background fluorescence which makes it ideal for observing low levels of fluorescence. It is highly stable, water white, and non-hydroscopic. Type FF can be substituted for all other fluorescence microscopy immersion oils in nearly all fluorescence applications.

OPTICAL & PHYSICAL PROPERTIES

Immersion Oil  Type LDF Type FF Type HF
Refractive Index @23°C
F Line (4861 A) 1.5239 1.4850 1.5234
E Line (5461 A) 1.5181 1.4811 1.5180
D Line (5893 A) 1.5150 1.4790 1.5150
C Line (6563 A) 1.5115 1.4766 1.5117
Dispersion:
nF-nC 0.0125 0.0084 0.0118
Abbe VD 41.3 57.0 43.8
Abbe Ve 41.0 56.5 43.6
Temperature Coefficient (15-35°C) -0.00036 -0.00037 -0.00038
Stability (change in nD25°C after 24 hrs @ temp.)
at 60°C: -0,0004 0 +0,0001
at 100°C: -0,0043 0 +0,0006
Fluorescence (1): Short UV: very low none very low
Fluorescence (1): Long UV: very low none very low
Color: (Gardner) <1 <1 <1
Viscosity: cSt +/- 10% @ 23°C (centistokes) 500
medium
170

low

700

medium

Density: @ 23°C g/cc: .984 .877 .9306
Density: (US) lb/gal 8.21 7.32 7.77
Cloud Point: <-14°C <-6°C <-2°C
Flash Point: 171°F 420°F 340°F
Neutralization No.: (mg KOH/g) <0.01 max 0.01 max 0.03 max

*Relative to Cedarwood Oil

Storage : RT

MSDS : Cargille-Immersion-Oil-Type-HF

Technical Data Sheet : Immersion-Oil-Type-HF

Additional information

Unit

1 oz, 4 oz, 16 oz

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