Carstairs Method for Fibrin and Platelets

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Description

FIXATION:        10% buffered neutral formalin for 48 hours or longer.

TECHNIQUE:        Paraffin sections cut at 5 μm.

SOLUTIONS:

Modified Weigerts Iron Hematoxylin

(See Gomori’s One Step Trichrome Method)

0.5% Acid Alcohol Solution

(See Truant’s Method for Acid Fast Bacilli)

Picro-Orange G Solution

Picric acid, saturated aqueous ————————————————-                100.0 ml

Orange G, C.I. 16230 ———————————————————–        1.0 gm

Ponceau-Fuchsin Solution

Acid fuchsin, C.I. 42685 ——————————————————-                0.5 gm

Ponceau 2R (Ponceau Xylidine) C.I. 16150 ————————————                0.5 gm

Distilled water ——————————————————————-        99.0 ml

Acetic acid ———————————————————————                1.0 ml

*Phosphomolybdic-Phosphotungstic Acid Solution

Phosphomolybdic acid ———————————————————-                2.5 gm

Phosphotungstic acid ———————————————————–                2.5 gm

Distilled water ——————————————————————-        100.0 ml

       *This solution is the same one used in Massons trichrome stain.

*Aniline Blue Solution

Aniline blue, C.I. 42755 ——————————————————–        –        1.0 gm

Distilled water ——————————————————————-        40.0 ml

Acetic acid ———————————————————————–        0.8 ml

       *This solution is the same one used in Massons trichrome stain.

Staining Procedure:

  • Use control slide.
  1. Deparaffinize and hydrate to distilled water.
  2. Modified Weigerts iron hematoxylin for 5 minutes.
  3. Wash in running water and rinse in distilled water.
  4. Place in acid alcohol for 5 seconds.
  5. Wash in running water for 1 minute and rinse in distilled water.
  6. Picro-orange G solution for 3 minutes.
  7. Rinse in three changes of distilled water.
  8. Ponceau-fuchsin for 3 minutes.
  9. Rinse in three changes of distilled water.
  10. Differentiate in phosphomolybdic acid-phosphotungstic acid solution for 3 minutes.
  11. Rinse in three changes of distilled water.
  12. Aniline blue solution for 2 minutes.
  13. Rinse in three changes of distilled water.
  14. Dehydrate in graded alcohols and clear in xylene, three or four changes.
  15. Mount with synthetic resin

Stain results:

  48 hours or more Less than 48 hours
Fibrin: Bright Red Orange to Orange-Red
Platelets Gray blue to navy Light Gray
Collagen: Bright Blue Bright Blue
Muscle: Red Red
Red Blood Cells: Clear Yellow Red, Green or Yellow

Additional information

unit

500 ml

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