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Description
MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe
To prepare 1 liter of MOPS Buffer (10X) (0.2 M, pH 7):
Table 1. Required components
| Component | Mass | Molarity |
| MOPS free acid (mw: 209.26 g/mol) | 41.86 g | 0.2 M |
| Sodium Acetate (mw: 82.03 g/mol) | 4.1 g | 0.05 M |
| Na2EDTA (mw: 372.24 g/mol) | 3.72 g | 0.01 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 41.86 g of MOPS free acid to the solution.
- Add 4.1 g of Sodium Acetate to the solution.
- Add 3.72 g of Na2EDTA to the solution.
- Adjust solution to desired pH using NaOH (typical pH = 7)
- Add distilled water until volume is 1 L.
storage : 0-5°C
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