Mops, Biological Buffer

(3-(N-Morpholino) propanesulfonic acid) pH range 6.5-7.9  

unit. 100 gr

MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced by Good et al. …

Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, 

MOPS is an excellent buffer for many biological systems at near-neutral pH.

2 in stock

REF: D18900 Categories: , , Tag:

Description

MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe

To prepare  1 liter of MOPS Buffer (10X) (0.2 M, pH 7):

Table 1. Required components
Component Mass Molarity
MOPS free acid (mw: 209.26 g/mol) 41.86 g 0.2 M
Sodium Acetate (mw: 82.03 g/mol) 4.1 g 0.05 M
Na2EDTA (mw: 372.24 g/mol) 3.72 g 0.01 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 41.86 g of MOPS free acid to the solution.
  3. Add 4.1 g of Sodium Acetate to the solution.
  4. Add 3.72 g of Na2EDTA to the solution.
  5. Adjust solution to desired pH using NaOH (typical pH = 7)
  6. Add distilled water until volume is 1 L.

 

storage : 0-5°C

MSDS_18900  

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