milloning phosphate buffer

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  Millonig’s Phosphate Buffer

  Millonig (1964); Karlsson and Schultz (1965)

  Ingredients: Sodium Phosphate, monobasic (NaH2PO4xH2O) Sodium Phosphate, dibasic (Na2HPO4xH2O) 0.5% Sodium Chloride (NaCl) Shipping Conditions: Ambient Temperature The pH of this buffer is 7.4 (0.1M);

  the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids.

   

  Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.

  Storage : 0-5°C                                            MSDS_11582  

  Ref: D11582

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