Negative EM Stain77

par Mamoun | Fév 3, 2022 | Info microscopy planet, Tips and tricks | 0 commentaires

Stain77 est un nouveau contrastant tres efficace en coloration négative .

il est à base de sel de lithium et de sels de tungsten à doses non toxique et un pH voisin du neutre . Le contraste se fait en bord de paillasse une minute suffit (sans plus de protection). Un lavage sur une goutte d’eau distillée 1 mn, secher puis observer.

nous l’avons testé sur des coupes ultrafines le contraste positif est efficace également tout comme l’UranyLess

Bacterie colloration négative et epithelium intestinal en coloration positive – coupe ultrafine

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Negative EM Stain77

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