Hall’s Method for Bilirubin

PRINCIPLE: The oxidizing action of Fouchet’s reagent converts the bile pigment to green biliverdin (if it has been transported to the liver and reduced, it is referred to as bilirubin.). Colors range from olive green to emerald green, depending on the concentration of bile pigment present.

Technical Data Sheet


MSDS 26611-01 MSDS 26611-02


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Staining Procedure:

  1. Deparaffinize and hydrate to distilled water.
  2. Stain in Fouchets reagent for 10 minutes.
  3. Wash in running water, then in distilled water.
  4. Stain in Van Giesons stain for 2 minutes.
  5. Dehydrate in 95% alcohol, three changes in Coplin jars.
  6. Complete dehydration in absolute alcohol, three changes.
  7. Clear in xylene, three or four changes.
  8. Mount with synthetic resin.


Stain results:

Biliverdin:   Green
Collagen:   Red
Muscle:   Yellow

Hall, M.J., Amer. J. Clin., 34:313 –316, 1960
Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Ed. McGraw-Hill, N.Y., p. 174, c. 1968


Informations complémentaires


250 ml, 500 ml


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