Transmission Electron Microscopes (TEM) transmit a beam of electrons through a sample to create an image. While the electrons pass through the sample, some will be scattered by heavier atoms in the sample creating a projection image. Imaging of biological material...
publication source from Po-Lin Chiu,Biological specimens have to be prepared for imaging in the electron microscope in a way that preserves their native structure. Two-dimensional (2D) protein crystals to be analyzed by electron crystallography are best preserved by...
Transmission electron microscopy (TEM) of rapidly frozen biological specimens, or cryo-EM, would benefit from the development of a phase plate for in-focus phase contrast imaging. Several types of phase plates have been investigated, but rapid electrostatic charging...
Water plays a central role in almost all life science experiments, such as preparing our samples with electron microscopy. It’s so important that there is even a high-impact journal dedicated to new methods of research and purification.The different laboratory...
SummaryCryogenic transmission electron microscopy of high-pressurefreezing (HPF) samples is a well-established technique for theanalysis of liquid containing specimens. This technique enables observation without removing water or other volatile components. The HPF...
Visualizing whole cells at many scalesCells need to compartmentalize thousands of distinct proteins, but the nanoscale spatial relationship of many proteins to overall intracellular ultrastructure remains poorly understood. Correlated light and electron microscopy...
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